Journal of Medicinal and Chemical Sciences

The Effectiveness of Epigallocatechin Gallate (EGCG) Eye Drops to Interleukin -1ß and Matrix Metalloproteinase (MMP)-9 Expression in Pseudomonas Aeruginosa Keratitis: An In Vitro Approach

Tarosa Yodia Urolita; Nurwasis .; Ismi Zuhria; Fariztah Sukainah Nur Fathimah; Annisa Karima; Nila Kurniasari; Djoko Agus Purwanto; Eko Budi Koendhori

Volume 7, Issue 3 , March 2024, , Pages 461-473

https://doi.org/10.26655/JMCHEMSCI.2024.3.1

Abstract

Introduction: Pseudomonas aeruginosa keratitis is the most prevalent eye infection, known to potentially lead to endophthalmitis and corneal perforation. Although antibiotic therapy ... Read More Introduction: Pseudomonas aeruginosa keratitis is the most prevalent eye infection, known to potentially lead to endophthalmitis and corneal perforation. Although antibiotic therapy effectively eliminates the bacteria, the damage induced by toxins in the cornea persists, resulting in corneal thinning, perforation, or fibrosis. The healing process of the corneal wound is influenced by Interleukin-1β and matrix metalloproteinase-9 (MMP-9). Epigallocatechin gallate (EGCG), possessing antibacterial, anti-inflammatory, and antioxidant properties found in Camellia sinensis extract, holds promise as an adjuvant therapy for bacterial keratitis. This study aims to analyze the expression of Interleukin-1β and matrix metalloproteinase-9 (MMP-9) in a keratitis model induced by Pseudomonas aeruginosa infection following the administration of Epigallocatechin Gallate (EGCG) eye drops (an experimental study conducted on Rattus norvegicus rat models).Methods: The experimental study utilized samples of Rattus norvegicus corneas, previously induced to form keratitis models, with 6 samples each in the negative control group, Moxifloxacin group, and combined Moxifloxacin and EGCG group. Results: The percentage of Interleukin-1β expression in the combined EGCG and Moxifloxacin group was lower compared to the Moxifloxacin group. The results indicated a significant difference in the Interleukin-1β expression among the test groups (p < 0.05). The post hoc test revealed significant differences between the Moxifloxacin group and the combined EGCG group (p < 0.05), as well as between the negative control group and the combined EGCG group (p < 0.05). The mean percentage of MMP-9 expression in the combined EGCG group was lower than that in the Moxifloxacin group. The One-way ANOVA test indicated a significant difference among the three treatment groups (p < 0.05).Conclusion: There was a significant reduction in the expression of Interleukin-1β and MMP-9 in animal models of keratitis induced by Pseudomonas aeruginosa infection following the administration of Epigallocatechin Gallate (EGCG) eye drops.

Effect of Platelet Rich Fibrin Membrane (PRF) or Conjunctival Autograft on VEGF Expression and Microvascular Density Post Conjunctival Excision

Aulia Ramadhan; Lukisiari Agustini; Dicky Hermawan; Ismi Zuhria; Reni Prastyani; Hari Basuki Notobroto; Djoko Legowo

Volume 7, Issue 2 , February 2024, , Pages 374-382

https://doi.org/10.26655/JMCHEMSCI.2024.2.9

Abstract

The conjunctiva has a significant role in maintaining the health of the eye's surface, particularly the cornea. Preserving the integrity of the conjunctival tissue is essential, as ... Read More The conjunctiva has a significant role in maintaining the health of the eye's surface, particularly the cornea. Preserving the integrity of the conjunctival tissue is essential, as extensive damage, coupled with inadequate treatment, can lead to a spectrum of eye disorders, ranging from mild conditions to severe cases resulting in blindness. Various techniques are available for repairing such conjunctival damage, including conjunctival autograft, amniotic membrane transplantation (AMT), oral mucosal graft, and the latest innovation, the use of PRF (Platelet Rich Fibrin) membrane. Nevertheless, it has remained unclear whether there are distinct effects on VEGF expression and angiogenesis following conjunctival excision when comparing PRF membrane grafts to conjunctival autografts. This study employs a true experimental design, utilizing a randomized post-test only two-group design, to compare the impact of PRF membrane transplantation and conjunctival autografting on VEGF expression and angiogenesis subsequent to conjunctival excision in adult New Zealand white rabbits. Twenty adult male rabbits were randomly split into two categories: the autograft cohort and the PRF membrane cohort. Conjunctival excisions were performed on the temporal conjunctival aspect of the right eye in each rabbit. In the first group, the conjunctival defect was repaired through conjunctival autografting, while in the second group, the defect was closed using a PRF membrane. After a period of 14 days following treatment, all samples were collected, and the right eyes that had undergone treatment were enucleated. The conjunctival tissues from the treated areas were then processed for histopathological examination. Immunohistochemical and Hematoxylin and Eosin (HE) staining were conducted on each sample. The Independent T-Test used for statistical analysis demonstrated a notably elevated VEGF expression level in the PRF membrane group in comparison to the conjunctival autograft group (p<0.05). Additionally, a significant variance in angiogenesis was evident between the PRF membrane and the conjunctival autograft groups (p<0.05).

Anti-Inflammatory Effect of Epigallocatechin Gallate on Tumor Necrosis Factor-Alpha and Interleukin-10 Expression in Pseudomonas aeruginosa-Induced Keratitis

Fariztah Sukainah Nur Fathimah; Tarosa Yodia Urolita; Annisa Karima; Ismi Zuhria; Delfitri Lutfi

Volume 7, Issue 1 , January 2024, , Pages 250-261

https://doi.org/10.26655/JMCHEMSCI.2024.1.24

Abstract

Pseudomonas aeruginosa is the most prevalent cause of keratitis, which can cause progressive corneal injury, scarring, thinning and corneal perforation. Antibiotic treatment alone is ... Read More

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Author = Zuhria, Ismi

The Effectiveness of Epigallocatechin Gallate (EGCG) Eye Drops to Interleukin -1ß and Matrix Metalloproteinase (MMP)-9 Expression in Pseudomonas Aeruginosa Keratitis: An In Vitro Approach

Abstract

Effect of Platelet Rich Fibrin Membrane (PRF) or Conjunctival Autograft on VEGF Expression and Microvascular Density Post Conjunctival Excision

Abstract

Anti-Inflammatory Effect of Epigallocatechin Gallate on Tumor Necrosis Factor-Alpha and Interleukin-10 Expression in Pseudomonas aeruginosa-Induced Keratitis

Abstract